TsHSP70 that metal ions conjugated with SO4

TsHSP70 and 100 genes were significantly up
regulated; ROS production and photosynthetic efficiency were adverse affected
when the T. suecica expose to HMs
especially the metals conjugated with SO42- anion. Overall,
expression levels of both genes were much higher in SO42-conjugated
metals than Cl- linked forms, showing that the TsHSP gene expression should be affected by metal and its conjugated
anion. This kind of transcription expression pattern was found in the dinoflagellate
P. minimum (Guo et al. 2012b), in the marine diatom Ditylum brightwellii (Guo et al. 2013) and a coral Montastraea franksi under exposure to redox-active
metal pollutants copper (Venn et al. 2009). In addition, in the marine diatom Ditylum brightwellii when they exposure metals conjugate with
SO4 and Cl2, the metals join with SO4 anion
showed increased gene expression when compared to Cl2 based
chemicals (Guo et al. 2013). Moreover, similar results were found in microalgae P.
minimum by using the stress-responsive gene
catalase-peroxidase (KatG) (Guo and Ki 2013).
These data suggest that HSPs are
differentially involved in defense against various metal stressors. From the
previous findings and our result it is important point needs to be considered;
it seems that anionic conjugates of metal (SO4, Cl2, etc.) may be responsible for
specific induction of HSP genes. This can be observed from the fact that metal
ions conjugated with SO4 were able to modulate the transcription of HSP70 and 100; however this was not the case for metals conjugated with Cl2. In another point of view, it might be explained by considering
the biological end points as one of the indicators for the bioavailability (or
susceptibility) of metals in phytoplankton (Campbell et al. 2002). However, the
copper used in the present study are conjugated compounds (CuSO4 and
CuCl2), and potentially dissolve differentially in the filtered
seawater medium. In
addition, they could react with the chelating agents EDTA present in the f/2
medium. Similar metal reaction could also explain the behavior of another
divalent metal viz., cadmium. Thus,
the bioavailability or susceptibility of metal salts may differently affect the
toxicity and stress gene expression in the marine green algae T. suecica (Guo et al. 2012b).


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