The present worke reports that syntheisis and characterization of silver nanoparticles (AgNPs) using papaya leaf extract (PLE) for treatment of epithelial cancer cells .The result of this work are synthesized nobel AgNPs-PLE exhibits anticancer activity by downlegulating the expression of cyclin D1 and increased the expression of p21,p27 as wells as p53 on DU-145 cells.Together, this study for the first time report that syntesized AgNP-PLE shown anticancer efficacy in various cancer cells and associated biological event in prostate cancer cells.The eco-friendly and cost-effective synthesis of AgNP-PLE provides therapeutic option for the treatment of prostate cancer patients. prostate cancer cells and it srole in cancer cells.