Home Research PapersPowdered leaves were extracted with cold acetone

Powdered leaves were extracted with cold acetone

Powdered leaves were extracted with cold acetone (80% + 20% water) several times. Chlorophylls a, b, total chlorophyll, and carotenoids were measured spectrophotometrically (Model Shimadzu UV- 1601, Shimadzu Corp., Japan) at 644.8, 661.6 and 470 nm following the method described by Lichtenthaler (1987). Chlorophylls a, b, total chlorophyll, and carotenoids were calculated as mg/g FW.
Phenolic compounds were assayed with Folin-Ciocalteu reagent as described by Chen et al. (2008). The calculations were based on a standard curve obtained with gallic acid. The content of phenolic was expressed as mg of gallic acid equivalent per g of fresh weight.
The activity of catalase (CAT; EC 1.11.1.6) in leaf was assayed by following the decomposition of H2O2 at 240 nm for 2 min. The reaction compound included potassium phosphate buffer (50 mM, pH=7.5, 4°C), 15 mM H2O2 and enzyme extract (10-20 ?L) in a total volume of 2 ml (Aebi, 1984).
Quantification of fruit biochemical quality, yield estimation, and water use efficiency
Yield water use efficiency (WUEET; kg/m3), was carried out using the water efficiency parameters (total fresh fruit yield/water amount consumed).

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