In recent studies revealed that human intestinal lactic flora produced multiple functional metabolites i.e. BSH, Bacteriocin, and EPS to strongly influence the success for the base of probiotic attributes (Maria et al., 2006 ;Messaoudi et la., 2013; Ren et al., 2014., ). Among these EPS have wide attention in industrial application with technological properties to health benefits to consumers (Wang et al., 2008; Ito et al., 2011). General forms of EPS existed as branched or linear in the various origins of plant, algae, fungi and bacteria (Wang et al., 2008). Usually, microbial EPS had predominately composed of D-glucose, D-galactose, D-mannose, D-xylose and D-arabinose moreover additional rare sugar exists in minor composition as D-ribose, fucose, glucosamine, galactosamine, galactouronic acid and glucouronic acid (Han et al., 2014 ; Li and Shah, 2016; Bramhachari and Dubey, 2006). That EPS develops their biological and functional properties to attractive in industries (Rani et al., 2017). As LAB had EPS from probiotics as commonly attained as GRAS (Generally Recognized As Safe) by its confirming of prolonged colonization and prebiotics in GI-tract (Gardiner et al., 1999; Welman and Maddox, 2008). Additionally, EPS from LAB had been presented to numerous health-promoting effects such as immunomodulating, antitumour, antioxidant and cholesterol-lowering properties (Li et al., 2015).In general, EPS are of two forms during cell growth as excreted and both excreted and cell bound by specific LAB (Li et al., 2015). EPS are separated into class as homo and heteropolysaccharide by its composition and biosynthetic mechanism. Homopolysaccharides are consist of one type monomeric units and it can synthesis large amount from the substrate of sucrose by the action of sucrase enzyme whereas heteropolysaccharides composed of multiple sugars synthesized by different glycosyltransferase enzymes (Van Hijum et al., 2006; De Vuyst and Degeest, 1999). In homopolymer of glucan showed viscosifying, stabilizing, emulsifying, sweetening, gelling, water binding and prebiotic agent in the food (Whitfield, 1988; Welman and Maddox, 2008; Das et al.,2014). In glucan produced Enterococcus spp from human gut reported limited and also it possess strong therapeutic with food application such as emulsifying, antimicrobial (Bajaji et al., 2015). To date, Enterococcus hirae have been investigated as probiotics from animal and dosa batter and also beneficial to human of high resistance to colonization against Salmonella enterica ser. Typhimurium than L. ruminis when administrated with in mice model (Gupta1&Tiwari1, 2015; Arokiyaraj et al., 2014; Galvão1 et al., 2017). Also in chemotherapy studies, it high expression immune cells and retain after antibiotic administration (Daille`re et al., 2016). Henceforth there is no report on EPS production and probiotic studies from Enterococcus hirae present in human origin.