A new method was established for estimation of Clobazam by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Clobazam by using Kromosil C18 4.5×150 mm 5.0 µm, flow rate was 0.8ml/min, and mobile phase ratio was 75:35% v/v ACE: di-potassium hydrogen phosphate, detection wavelength was 532 nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.482 mins. The % purity of Clobazam was found to be 99.87%. The system suitability parameters for Clobazam such as theoretical plates and tailing factor were found to be 4146, 1.23, the. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Clobazam was found in concentration range of 30µg-150µg and correlation coefficient (r2) was found to be 0.997, % recovery was found to be 100.4%, %RSD for repeatability was 0.5, % RSD for intermediate precision was 1.0. The precision study was precision, robustness and repeatabilty.LOD value was 2.97 and LOQ value was 9.92.Hence the suggested RP-HPLC method can be used for routine analysis of Clobazam in API and Pharmaceutical dosage form.